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Application

Critical parameters of the sample application are:

Critical parameters of the sample application are:

  • The precise position of the sample: Chromatography must start simultaneously for all samples on a plate to ensure comparability of RF of separated zones. For that, a standardized plate layout is adopted.
  • The application volume: Precise application volumes are prerequisite for qualitative and quantitative analysis. A common problem is sample overloading, which decreases the separation power and causes tailing of zones.
  • No damage to the layer: Damages to the layer during sample application can affect the movement of the mobile phase. Do mark the application position on the layer.

Other parameters are discussed in more detail in the following sessions:

Contact or spray-on?
Band or spot?
Plate layout
Return to Section 2. “HPTLC parameters”

Reference:

Reich E, Schibli A. High-performance Thin-layer Chromatography for the Analysis of Medicinal Plants. 2007. New York, NY: Thieme; 264 pages. ISBN-13: 978-1-58890-409-6.