Based on the intended DBS application that a laboratory wants to perform, the instrumental steup must be adjusted.
Standard LC-MS/MS applications
Basic DBS-LC-MS/MS methods require the following:
- CAMAG DBS-MS 500 or CAMAG DBS-MS 500 HCT
- Binary LC pump to run a gradient.
- Column oven
- Mass spectrometer
Using this setup, the analysis of drugs of abuse, TDM etc. is possible.
Online-SPE-LC-MS/MS applications
More advanced DBS-Online-SPE-LC-MS/MS methods require the following:
- CAMAG DBS-MS 500 or CAMAG DBS-MS 500 HCT
- 2x Binary LC pump to run a gradient.
- Column oven
- Mass spectrometer
- switching valve (e.g. 6/2 or 10/2)
Using this setup, the analysis of Phosphatidylethanol (PEth) is possible.
Offline DBS applications
For the offline sampling of DBS into sample vials or microtiter plates (e.g. 96-well plates) the following equipment is required:
- CAMAG DBS-MS 500 or CAMAG DBS-MS 500 HCT
- CTC PAL autosampler with a dilutor tool, a sample rack and a needle wash station, optionally The CTC PAL can be upgraded with an active sample wash station and a peltier stack for storing the extracts at 4°C
Using this setup, further manual sample processing (e.g. ELISA) is possible.
